Custom Synthesis

Peptide Discovery Synthesis

Accelerate Peptide Synthesis with Our Strong Chemistry Capability

WuXi TIDES peptide discovery platform provides library screening and cusom synthesis to address your needs and offer flexible options for various scales, purity levels, modifications requirement.

In 2024, we provided discovery peptide services for 500+ customers globally and delivered 41,000+ peptides from mg to kg scale.

We support the following peptide modalities, including but not limited to:

• Long Linear Peptide
• Cyclic Peptide
• Modified Peptide
• PEGylated Peptide
• Peptidomimetic

Service Highlights

Rich Experience

linear peptide up to 200 AAs, macrocyclic peptide, modified peptide and peptide based complex conjugates

Broad Scale

scale from mg to kg purity up to 99.9%

High Success Rate

over 99% on time delivery

Comprehensive Analysis Support

default purity/MS profile and additional services

High-Throughput Peptide Synthesis

up to 1,000 peptides synthesis in parallel

Quick Turnaround Time

2 weeks turnaround time for most peptides under 30 AA at mg scale

Deliverables

Form

• Lyophilized powder
• Stock solution

Certificate of Analysis

• HPLC/UPLC and LCMS
• ID, barcode of vials
• Sequence/structure
• Quantity, purity
• Storage condition, etc.

Packaging and Logistics

• Nitrogen packaging available
• Ambient logistics worldwide
• With icebag
• Dry ice logistics with optional thermal control

Note: Additional Services (TFA removal, analytical HPLC, etc.) are available at additional cost

Library Synthesis

We support both parallel and sequential synthesis with various high-throughput synthesizers with up to 192 channels and microwave synthesizers enabing AA coupling as fast as 5 min/AA.

| Item | <10 AAs | 10-20 AAs | 20-30 AAs | |---|---|---|---| | Number of Peptides | 48 peptides in parallel synthesis | | | | Delivery Time | <8 days | <12 days | <20 days | | ▸ Peptide Companion Prediction-assisted assessment can be applied prior to automated synthesis. ▸ Insoluble (and/or expensive) AAs (building blocks) are not recommended for automated synthesis. ▸ The microwave-assisted synthesizer excels in facilitating difficult peptide synthesis. ▸ The combination of automated and manual synthesis is employed to expedite the process of long peptide synthesis and enhance the success rate. | | | |

With ~20 various high throughput synthesizers, we will support peptide library synthesis from 10 µmol – 1.0 mmol. We can synthesize peptide libraries up to 800 peptides in parallel with high success rate up to 96%. Our strong analytical support ensures high quality products with the capability to reach up to 99.9% purity.

Our team has in-depth experience with 50,000+ linear peptides and 100,000 cyclic peptides and modifications in the past years.

Automatic Peptide Synthesizer

We have 20+ high throughtput synthesizers, supporting the scale ranging from 10 µmol – 1.0 mmol.

CEM Liberty Blue

• Sequential synthesis
• 12-channel
• 25 µmol - 0.2 mmol

Biotage Syro II

• Parallel synthesis
• 36/48/96/192 channel
• 10 µmol - 0.2 mmol

CSBio Synthesizer

• Parallel synthesis
• 3-channel
• 0.2-0.5 mmol

Custom Synthesis

Linear Peptide Synthesis

Approach:

• Fmoc Solid-Phase Peptide Synthesis (SPPS)
• Fragment Coupling
• Ligation

Experience Highlight

• Length up to 250 AAs
• More than 50,000 linear peptides and modifications delivered
• More than 10,000 complex peptides delivered, including PNAs, peptoids, branched peptides, dendrimers, peptidomimetic peptides

Cyclic Peptide Synthesis

Cyclization is a prevalent strategy employed to enhance the pharmacological properties of a peptide from its linear precursor. These enhancements may include improved affinity and specificity, proteolytic stability, and/or solubility.

Our teams possess a wealth of experience in cyclic peptide synthesis, successfully delivered over 100,000 cyclic peptides.

We utilize a variety of cyclization strategies such as single/double/triple disulfide, click cyclization, lactone, lactam, and RCM. The possible cyclization positions include head-to-tail, head-to-sidechain, sidechain-to-sidechain, and sidechain-to-tail.

Head to Tail

Head to Sidechain

Sidechain to Sidechain

Sidechain to Tail

Experience in:

• Disulfide Peptide (single/double/triple disulfide)
• Thioether Cyclization
• Lactam Cyclization
• Lactone Cyclization
• Click Peptide
• RCM Cyclization
• Bicyclic Peptide

Bicyclo-disulfide Bridge

(three pairs of S-S bond formation achieved as well)

RCM Monocyclic Peptide

Bicyclic Peptide

Click Peptide

Thioether Cyclization

Modified Peptide Synthesis

We offer a comprehensive range of specialized modifications to meet your specific requirements. These include N-terminal modifications, C-terminal modifications, dye/fluorescent labeling, biotin-labeling, PEGylation, post-translational modifications, DOTA/NOTA labeling, and isotope labeling.

Beyond sidechain and backbone modifications, we are also capable of incorporating unnatural amino acids into the sequences. For more information on our catalog of unnatural amino acid products, please visit here.

Modified Peptide Synthesis

N-terminal Modification

C-terminal Modification

Dye and Fluorescent (site specific modification)

PTMs (site specific modification)

Isotope Labeling

Glycopeptide

N-terminal Modification

• H (free amine)
• Acetylation
• Fmoc
• CBZ
• Bz
• Pyr
• Biotinylation
• Lipid tail
• Dye and fluorescent labeling

C-terminal Modification

• -OH (free)
• Ester (-Ome)
• -NH2
• -NH-Me
• Active ester (NHS; TFP; PFP)
• Dye and fluorescent (by conjugation)

Dye and Fluorescent (site specific modification)

• Biotin；
• FITC；
• 5-FAM (5,6-FAM)；
• Dansyl-
• TAMTA-
• MCA-
• Rhodamine

PTMs (site specific modification)

• Acetylation (Lys)
• Methylation (Arg)
• Phosphorylation (Ser, Thr, Tyr)
• Lipidation
• Glycosylation
• SUMOylation
• Ubiquitionation

Isotope Labeling

• 1 H – D
• 12 C – 13 C
• 14 N – 15 N
• 13 C, 15 N – Lys
• 13 C, 15 N – Arg
• 13 C, 15 N – Ala
• 13 C, 15 N – Ile
• 13 C, 15 N – Leu
• 13 C, 15 N – Pro
• 13 C, 15 N – Phe
• 13 C, 15 N – Val

Glycopeptide

• N-linked glycopeptide synthesis
• O-linked glycopeptide synthesis
• C-linked glycopeptide synthesis
• S-linked glycopeptide synthesis

Advanced Peptide Synthesis

We support the following advanced peptides.

• Ligated Peptide
• Peptidomimetics
• Peptoid
• PNA
• Branched Peptides
• Dendrimers, and more

Various Reactions on Solid Support

• Suzuki Coupling
• Sonogashira Coupling
• Heck Reaction
• Reductive Amination
• Ugi Reaction
• Wittig Reaction
• Hoffman Degradation
• Curtis Rearrangement
• Reduction/Oxidation, and more

Related Resources

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Peptide CRDMO Chemistry Platform

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How Does It Work? Peptide Oligonucleotide Conjugate

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Find more resources

WuXi AppTec does not provide bacteriophage display, mRNA display, or ribosomal display-based screening services for bicyclic peptides constrained on a molecular scaffold. WuXi AppTec does provide a variety of DNA-Encoded Library (DEL) screening services.